ANP32B promotes colorectal cancer cell progression and reduces cell sensitivity to PRAP1 inhibitor through up-regulating HPF1.

ANP32B, a member of the acidic leucine-rich nuclear phosphoprotein 32 family member B, is aberrantly expressed in various cancers, including colorectal cancer. However, the function and mechanism of action of ANP32B in colorectal cancer remain unclear. The present study therefore analyzed the expression of ANP32B and its activity in colorectal cancer patient samples and colorectal cancer cell lines. ANP32B expression was found to be significantly upregulated in colorectal cancer patient samples and cell lines. Upregulation of ANP32B enhanced colorectal cancer cell proliferation and migration, whereas downregulation of ANP32B suppressed colorectal cancer cell proliferation. RNA sequencing analysis of differentially expressed genes in ANP32B silenced colorectal cancer cells showed that histone PARylation factor 1 (HPF1), which protects against DNA damage by interacting with the anti-tumor target PARP1, was significantly downregulated. Luciferase promoter assays testing the regulatory association between ANP32B and HPF1 showed that ANP32B interacted with the HPF1 promoter. Analysis of colorectal cancer samples from The Cancer Genome Atlas showed that ANP32B and HPF1 expression were positively correlated, and recovery assays showed that ANP32B promoted colorectal cancer progression by up-regulating HPF1. Overexpression of ANP32B also reduced the sensitivity of colorectal cancer cells to PARP1 inhibitor, consistent with the oncogenic role of ANP32B. ANP32B may alter the sensitivity of colorectal cancer cells to PARP1 inhibitor via a mechanism associated with the HPF1 gene. In summary, these findings showed that ANP32B acted as a tumor promoter, potentiating both colorectal cancer malignancy and drug resistance. Targeting the ANP32B/HPF1 axis may have benefit for patients with colorectal cancer.

Solid waste filling and roadway retaining for longwall mining by numerical investigation.

Solid waste filling and roadway retaining can effectively control surface subsidence and alleviate solid waste accumulation pollution. In order to effectively evaluate the advantages of solid waste filling in deformation control of overlying strata and surrounding rock of retained roadways, this study used theoretical analysis and numerical simulation methods to analyze the factors affecting surface subsidence, as well as the deformation characteristics of surrounding rocks and retaining tunnels during backfill mining. By calculating the influence of factors such as the foundation coefficient and the filling rate on the subsidence of the roof, it is concluded that the filling rate is the main controlling factor affecting the subsidence of the roof. Through simulation and comprehensive analysis of the impact of different filling rates on overlying rock migration, it was found that when the filling rates are 70 % and 80 %, it can effectively control the subsidence of overlying rock in the mining area. By simulating the effects of these two filling rate conditions on the deformation of surrounding rock within the retained roadway zone, the results show that the optimal filling rate that can effectively control the subsidence of the overlying rock and improve the stability of the retained roadway is 80 %.